ABSTRACT
Avaliou-se o efeito protetor das frações proteicas do soro do leite sobre as vilosidades intestinais de camundongos Balb/C, fêmeas, infectadas por Escherichia coli O157:H7. Foram utilizados 48 animais, distribuídos aleatoriamente em oito grupos de seis fêmeas cada um. Os animais dos grupos 1 e 2 (controles) receberam dieta AIN93G padrão; os dos grupos 3 e 4, AIN93G + alfalactalbumina; os dos grupos 5 e 6, AIN93G + betalactoglobulina e os dos grupos 7 e 8, AIN93G + concentrado proteico total e água ad libitum por sete dias. No dia zero, os animais dos grupos 2, 4, 6 e 8 foram inoculados, por meio de cânula de gavagem, com 0,5mL de E. coli O157:H7, na concentração de 7 x 10(10)UFC/mL. Os animais foram acompanhados clinicamente e sacrificados, no oitavo dia experimental. Verificou-se, por meio de exames histológicos e da morfometria, que as frações betalactoglobulina e alfalactalbumina exerceram efeito protetor sobre as vilosidades intestinais do jejuno distal e do íleo (P<0,05), respectivamente. O concentrado proteico total não demonstrou efeito protetor sobre as vilosidades intestinais.
The protective effect of protein fractions of whey on intestinal villi of Balb/C female mice infected with Escherichia coli O157: H7 was evaluated. A total of 48 animals were randomly distributed into eight groups of six females each. Animals in groups 1 and 2 (controls) received AIN93G standard diet; the groups 3 and 4, AIN93G + alpha-lactalbumin; while groups 5 and 6, AIN93G + beta-lactoglobulin; and groups 7 and 8, AIN93G + total protein concentrate and water ad libitum for seven days. On day zero, animals of groups 2, 4, 6, and 8 were inoculated by gavage tube with 0.5mL of E. coli O157: H7 at a concentration of 7 x 10(10)CFU/mL. The animals were clinically followed and sacrificed on the eighth day. It was verified by histological examination and morphometry that the beta-lactoglobulin and alpha-lactalbumin exerted a protective effect on the villi of the distal jejunum and ileum (P<0.05), respectively. The total protein concentrate showed no protective effect on the villi.
Subject(s)
Animals , Escherichia coli/pathogenicity , Milk Proteins/therapeutic use , Ileum/anatomy & histology , Jejunum/anatomy & histology , Protective AgentsABSTRACT
The antimicrobial resistance profile of 45 Staphylococcus strains isolated from 10 samples of Brazilian type "coalho" cheese was evaluated against eight antibiotics used either in human or in veterinarian medicine. The tested antibiotics decreasing resistance degree was: penicillin (100.0 percent), tetracycline (91.0 percent), vancomycin (75.5 percent), gentamicin (71.1 percent), oxacillin (66.7 percent), erythromycin (60.0 percent), cephalothin (48.9 percent) and sulphazothrin (26.7 percent). The high frequency of Staphylococcus strains presenting resistance to the tested antibiotics, emphasizes the importance of the control of the abusive use of antibiotics by medical and veterinarian subjects.
Subject(s)
Cheese , Drug Resistance, Bacterial , Microbial Sensitivity Tests , StaphylococcusABSTRACT
Os autores analisam uma serie de 90 casos de nodulo pulmonar estudados por tomografia axial computadorizada. Demonstram a grande importancia da densitometria nestas lesoes, permitindo a deteccao de depositos de calcio em granulomas, nao demonstrados por quaisquer outros metodos radiologicos.Os tumores mostraram valores distintos dos granulomas residuais. As lesoes tuberculosas ativas apresentaram caracteristicas proprias, devidas principalmente a presenca de material caseoso liquefeito